- 作者: 張錫祺
- 作者服務機構: 臺灣省立屏東農業專科學校
- 中文摘要: Five strains of Newcastle Disease Virus (NDV) were isolated from ducks insouthern Taiwan. Among them, three strains were isolated from 74 cases of Mule(Tofon) ducks and two strains were isolated from 34 cases of Common (Tnorsh)ducks, but none from 29 cases of Muscovy ducks. Those five NDV strains (CH, KF,PP, PD, and PT strain) were undergone 3 passages in chicken embryo for the studyof their character. B and Sato strain were used as controls. The experimentalresults were summarized as follows: (1) The TCID /0.1m1 were determined as follows: 10 for B strain, 10 for CHstrain, 10 for KF strain, 10 for PP strain, 10 for PD strain, 10 for PT strain,and 10 for Sato strain. (2) The MDT were determined as follows: 144 hrs for B strain, 56 hrs for CHstrain, 64 hrs for KF strain, 64 hrs for PP strain, 56 hrs for PD strain, 56 hrs forPT strain, and 56 hrs for Sato strain. (3) The ICPI were determined as follows: 0.16 for B strain, 1.64 for CH strain,1.64 for KF strain, 1.69 for PP strain, 1.61 for PD strain, 1.73 for PT strain, and 1.66for Sato strain. (4) The IVPI were determined as follows: 0 for B strain, 2.07 for CH strain,1.95 for KF strain, 2.06 for PP strain, 1.99 for PD strain, 2.11 for PT strain, and 2.13for Sato strain. (5) The pathogenicity of B , CH, and PT strain in laying hens were determined.There were no pathogenesis for laying hens which were inoculated with B strain,but death occurred in all laying hens within 5-7 days which were inoculated withCH and PT strain. (6) Transmissibility of the virus from artificially infected ducks to ND freechickens was occurred when housed in the same cages; the results were that allchickens were died within 10-13 days, therefore this result show that transmissibilityis possible. From the above experimental results, we can understand: that five strains of NDV isolated from ducks in the field were all belong to Velogenic type, ducks were less sensitive to NDV, but the excretion of NDV in highly infected ducks could transmit it to chickens.
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