- 作者: Xian Wang; Kenneth KK Lau; Leo KY So; Yun Wah Lam
- 作者服務機構: Department of Biology and Chemistry, City University of Hong Kong, Hong Kong SAR, Mainland China
- 中文摘要: --
- 英文摘要:
Background :Nonhistone chromosomal proteins in concert with histones play important roles in the
replication and repair of DNA and in the regulation of gene expression. The
deregulation of these proteins can contribute to the development of a variety of
diseases such as cancer. As a nonhistone chromosomal protein, chromodomain
helicase DNA binding protein 5 (CHD5) has recently been identified as the product of
a novel tumor suppressor gene (TSG), promoting the transcription of p19ink4a and
p16arf. The inactivation of CHD5 was achieved partly through genetic deletion since it
is located in 1p36, a region frequently deleted in human tumors. In this study, we aim
to study the involvement of CHD5 in gastric cancer, the second most common cancer
worldwide.
Methods :
CHD5 expression in a panel of gastric cancer cells were determined by quantitative
RT-PCR. The methylation of CHD5 was evaluated by methylation specific PCR and
bisulfite genome sequencing. The effect of CHD5 on growth of gastric cancer cells
was tested by colony formation assay.
Results :
CHD5 expression was down-regulated in all of gastric cancer cell lines used (100%,
7/7) and significantly restored after pharmacological demethylation. Methylation of
CHD5 promoter was detected in all of seven gastric cancer cell lines and in the
majority of primary gastric carcinoma tissues examined (73%, 11/15). Finally, ectopic
expression of CHD5 in gastric cancer cells led to a significant growth inhibition.
Conclusions : CHD5 was a TSG epigenetically down-regulated in gastric cancer. - 中文關鍵字: --
- 英文關鍵字: --