- 作者: Rosa Huang Liu; Sun-Long Cheng; Kwo-Chang Ueng; Der-Jinn Wu; Shui-Tein Chen; Supachok Sinchaikul; Tsung-Han Lee
- 作者服務機構: 1 Department of Life Sciences, National Chung Hsing University, 250, Kuo-Kuang Road, Taichung, 40227, Taiwan; ; 2 Department of Surgical Medicine, Institute of Medicine, Chung Shan Medical University, Taichung, Taiwan; ; 3 Division of Cardiology and Cardiovascular Surgery, Institute of Medicine, Chung Shan Medical University, Taichung, Taiwan; ; 4 Department of Internal Medicine, Institute of Medicine, Chung Shan Medical University, Taichung, Taiwan; ; 5 Institute of Biological Chemistry and Genomics Research Center, Academia S璯ica, Taipei, Taiwan; ; 6 Institute of Biochemical Sciences, College of Life Science, National Taiwan University, Taipei, Taiwan
- 中文摘要: --
- 英文摘要: We used human DNA microarray to explore the differential gene expression profiling of atrial natriuretic peptide (ANP)-stimulated renal tubular epithelial kidney cells (LLC-PK1) in order to understand the biological effect of ANP on renal kidney cell's response. Gene expression profiling revealed 807 differentially expressed genes, consisting of 483 up-regulated and 324 down-regulated genes. The bioinformatics tool was used to gain a better understanding of differentially expressed genes in porcine genome homologous with human genome and to search the gene ontology and category classification, such as cellular component, molecular function and biological process. Four up-regulated genes of ATP1B1, H3F3A, ITGB1 and RHO that were typically validated by real-time quantitative PCR (RT-qPCR) analysis serve important roles in the alleviation of renal hypertrophy as well as other related effects. Therefore, the human array can be used for gene expression analysis in pig kidney cells and we believe that our findings of differentially expressed genes served as genetic markers and biological functions can lead to a better understanding of ANP action on the renal protective system and may be used for further therapeutic application.
- 中文關鍵字: --
- 英文關鍵字: atrial natriuretic peptide (ANP), LLC-PKl cells, gene expression, microarray