- 作者: 宋雅茹、陳曉霞、吳紅、卲會波
- 作者服務機構: Department of Chemistry, Capital Normal University, Beijing 100037, P. R. China
- 中文摘要: Stable films made from chitosan (CS) on pyrolytic graphite (PG) electrode gave direct electrochemistry for incorporated enzyme nitrate reductase (NR). Cyclic voltammetry (CV) of NR-CS films showed a pair of well-defined and nearly reversible redox peaks at about -0.430 V vs. SCE at pH 7.0 phosphate buffers, which was considered as the redox of FAD and heme-ion. The electron transfer between NR and PG electrode was greatly facilitated in CS films. Integration of reduction peaks at different scan rates from 0.01 to 0.5 V s-1 gave nearly constant charge values, which is characteristic of thin-larger-electrochemical behavior. The pH of the solution strongly affected the direct electron transfer of NR-CS films. EDTA accelerated the electron transfer, and it was proposed as a stimulant for the system. Reflectance absorption infrared spectra demonstrated that NR retained a nearly native conformation in CS films.
- 英文摘要: --
- 中文關鍵字: Nitrate reductase; Chitosan; Cyclic voltammetry.
- 英文關鍵字: --