• 作者： 劉雨田
• 作者服務機構： 國防醫學院微生物及免疫學科
• 中文摘要： 自Streptomyces nodosus，IMRU 3694菌株的突變中選出一株優良變種，定名為Streptomyces nodosus, NDMC-034，此變種的特性是產生芽胞的能力減低，失去產生黑色素（Melanoid pigment）的能力，並且在28℃培養時，其產生am-photericin A的能力增加。相反地，如在32℃培養則其amphotericin A的生產能力降低而amphotericin B的產量反而增加一倍，因此，其amphotericins的生物合成可以培養溫度加以控制。 另自Streptomyces griseus, IMRU 3570菌株的突變中分離出一株特殊的變種，其特性是幾乎完全失去生產candi-cidin的能力但其生長不受影響。證明candicidin的生合成與菌種的生長無直接關係。所有選出之變種皆以Auxanography的方法測其對生長因素的需求特性。
• 英文摘要： A novel mutant strain designated as Streptomyces nodosus, NDMC-034 was selected from the muta-tion of Streptomyces nodosus, IMRU 3694. This mutant was characterized by delayed sporulation, theincapability of producing the melanoid pigment either in the production medium or on YD agar containing1% yeast extract and 1% dextrose, and the increased yield of amphotericin A as compared to its parentstrain. In addition, the biosynthesis of amphotericin A by this mutant seems to be sensitive to temperature.When the incubation temperature was raised to 32℃, the yield of amphotericin A was dramatically decreas-ed; in contrast, the yield of amphotericin B was greatly increased as compared to that at 28℃. Another unique mutant strain designated as Streptomyces griseus, YT-1 was selected from the muta-tion of Streptomyces griseus, IMRU 3570. It was characterized by the incapability of synthesizing candici-din and the delayed sporulation. All the mutants were auxanographed and characterized by the requirement for growth factor.
• 中文關鍵字： polyene macrolide antibiotic; mutation; auxanography; amphotericin; nystation; candicidin; biosynthesis
• 英文關鍵字： --