- 作者: Yuan Xu; Thipparthi Raghavendar Reddy; Wolfgang H. Fischer; Folssie Wong-Staal
- 作者服務機構: a Department of Medicine and Biology,University of California at San Diego,La Jolla, Calif, ; b Clayton Foundation Laboratories for Peptide Biology, Salk Institute,La Jolla, Calif, USA
- 中文摘要: --
- 英文摘要: We have identified and obtained the full-length clone of RREBP49, a human nuclear factor which specifically interacts with the Rev-responsive element (RRE) sequence of human immunodeficiency virus type 1. Sequence analysis revealed that RREBP49 is highly homologous to hnRNP F protein and contains three repeated RNA-binding domains. Binding assays demonstrated that Rev and RREBP49 bind to different subregions on the RRE sequence and that binding is mutually nonexclusive. Blocking of endogenous RREBP49 expression by an antisense construct increases Rev activity in CV-1 cells, indicating that RREBP49 and Rev may play antagonistic roles in HIV-1 replication. RREBP49 may function as a splicing factor or a nuclear retention factor for unspliced mRNAs. However, only a slight decrease of Rev activity was observed when exogenous RREBP49 was introduced into CV-1 cells by pSVL-RREBP49 expression vector. This may be explained by a high endogenous level of RREBP49 which is above optimal. Alternatively, additional cellular factors may be required for RREBP49-mediated inhibition of Rev.
- 中文關鍵字: --
- 英文關鍵字: HIV-1 ; Rev protein; Rev-responsive element ; RREBP49 ; Ribonuclear protein, ; heterogeneous nuclear