第8卷‧第1期,
198001
, pp. 51-57
應用豬胚胎腎臟細胞株培養弓蟲之研究
- 作者：
張甘楠; 郭謨
- 作者服務機構：
臺灣省立屏東農業專科學校
- 中文摘要：
以豬胚胎腎臟細胞株（以下簡稱為 ESK 細胞）在試管內使弓蟲增殖的方法，本試驗係首次研究成功者。本試驗以增殖型弓蟲為 1 與 ESK 細胞數為5的比例培養結果能使弓蟲在 ESK 細胞質內行二分裂法增殖，且培養後之各種不同的時問弓蟲增殖之情況有各種不同的性狀，例如培養後1小時可發現弓蟲侵入 ESK 細胞質內，30小時弓蟲在 ESK 細胞質內形成薔薇花排列(rosette formation)；36小時可發現弓蟲在 ESK 細胞核四周圍成一圈而繼續其胞內二裂增殖(Endodyogeny) 之現象。72小時增殖的弓蟲數為原來弓蟲數的126倍之多。本試驗的優點為：(1)操作簡便，不似初代細胞培養及小白鼠腹腔注射接種之易於污染雜菌且費時費力。(2)經過繼代培養10代，該弓蟲的毒力不變。(3)本試驗似可提供在試管內對弓蟲之其他項目研究之參考。
- 英文摘要：
Serial passages of Toxoplasma gondii in vitrowere successfully performed in a kidney cellline derived from the embryonic swine (ESKcell line). The inoculation ratio of the parasiteto the host cell was one to five. Invationof the parasite into the host cell was readilydiscernible one hour thereafter. Proliferationof the parasite was then followed inside thecytoplasm of the host cell. Rosette formations,ordered aggregates of Toxoplasma, wereobserved in the cytoplasm 30 hours post inocula-tion. Reproducing parasite could also encirclethe nucleus of their host cell. The parasiteswas enriched 126 folds 72 hours after theculture. The toxicity of the Toxoplasma againstthe mouse was not attenuated even following 10serial passages through the BSK cell line. Theapplication of the Toxoplasma culture in vitrowas discussed.
- 中文關鍵字：
--
- 英文關鍵字：
--
