• 作者： 張勝祺; 盧青佑; 黎世源; 魏耀揮
• 作者服務機構： 國立陽明醫學院生化學科; 私立中國文化大學化學系
• 中文摘要： Eremofortin C（EC）及PR toxin是藍酪黴菌的次級代謝產物，兩者結構很相似，惟一不同點在第12碳上，前者為醇基而後者為醛基。EC已被證明是PR toxin之前趨物，且已證明可經由EC氧化酵素氧化為PR toxin。藍酪黴菌產生此兩個化合物之培養基配方一直採用15％蔗糖及2％酵母萃取物，近年來本實驗室發現在此培養基中添加玉米煮出液，能同時增加此兩個化合物之產量及提高EC氧化酵素之活性。藍酪黴菌在含7.5％蔗糖、1％酵母萃取物及20％玉米煮出液之培養基中，其產生EC、PR toxin最高量及EC氧化酵素最高活性，分別為不添加玉米煮出液的6.2倍、4.6倍及4.7倍。此外，玉米煮出液促進EC、PR toxin產量及EC氧化酵素活性之增加具有劑量關係。然而培養基在添加玉米煮出液後，若增加原有蔗糖及酵母萃取物之含量，則EC、PR toxin產量及EC氧化酵素活性反而降似。
• 英文摘要： Eremofortin C (EC) and PR toxin are secondary metabolites of Penicillium roqueforti. Theirstructures are similar and differ only by an alcohol and an aldehyde group at the C-12 position. EC hasbeen demonstrated to be the precursor of PR toxin, and EC is transformed to PR toxin by EC oxidase.These two compounds and EC oxidase are secreted by P. roqueforti in the culture medium, which is usuallycomposed of 15% sucrose and 2% yeast extract. Recently, we discovered that the addition of corn extractto this medium increased the production of EC and PR toxin and the activity of EC oxidase in a coordina-tive manner. In a time-course study, we found that the peak yield of EC and PR toxin and the maximum activityof EC oxidase in the culture medium containing 7.5% sucrose, 1% yeast extract, and 20% corn extract wereincreased 6.2, 4.6, and 4.7-fold, respectively, as compared with those obtained in the medium without cornextract· Moreover, corn extract increased the production of EC and PR toxin and the activity of ECoxidase by P. roqueforti in a dose-dependent manner. On the other hand, when the concentrations ofsucrose and yeast extract were increased while fixing the ratio of corn extract, we found that the levels ofEC and PR toxin and the enzyme activity were decreased concomitantly. We thus conclude that cornextract can enhance the production of EC, PR toxin and EC oxidase by P. roqueforti when grown in aminimal medium and that the potentiation effect of corn extract is suppressed when the fungi are grownin a rich medium.
• 中文關鍵字： eremofortin C; PR toxin; eremoforin C oxidase; Penicillium roqueforti.
• 英文關鍵字： --